Fig. 4

Expression of Capn5 is elevated in the adult XOPS:mCFP retina. (A) RT-PCR for capn5a and capn5b expression in WT and XOPS:mCFP retinas. Capn5a and capn5b are both expressed in the adult WT retina and capn5a levels are increased in the XOPS:mCFP retina. NT, no template control. (B) qPCR for capn5a and capn5b in WT and XOPS:mCFP adult retina; a 4-fold increase of capn5a expression was observed in the XOPS:mCFP retina compared with WT. (C–C′) FISH showing expression of capn5a in the adult (WT) retina; expression was seen in the IS of the cones. (D–D′) FISH showing expression of capn5a in the XOPS:mCFP retina; expression in the cone IS appears to be more intense than in WT. (E–E′) FISH for capn5b in the adult WT retina; modest expression was detected in the ISs. (F–F′) FISH for capn5b in the XOPS:mCFP retina; expression levels appears similar to WT. (G–G′) IHC for Capn5 (a, b) in the WT adult retina; expression was detected in the OPL and in the cone inner segments (IS). (H–H′) IHC for Capn5 in the XOPS:mCFP retina; increased expression was detected in the OPL and IS. Scale bars: 100 μm. *P < 0.05.