FIGURE

Fig. 1

ID

ZDB-FIG-180824-27

Publication

Gao et al., 2018 - IDH1-mutated transgenic zebrafish lines: An in-vivo model for drug screening and functional analysis

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Fig. 1

Characterization of Nes^IDH1 zebrafish lines.

Expression of the transgene was detected in the central nervous system (CNS) of zebrafish embryos using fluorescent imaging (A: non-transgenic wildtype zebrafish showing background auto-fluorescence staining, mainly in the yolk sac; A’: Nes^IDH1 show expression of the transgene in the CNS of 3dpf embryos). Expression was confirmed by immunochemistry staining using an anti-GFP antibody (B) and Q-PCR (C). D2HG only accumulated in Nes^R132H zebrafish (D, non-transgenic vs Nes^IDH1wt, p = 0.754, non-transgenic vs Nes^R132H, p = 0.003, student’s t-test). L2HG levels in the transgenic lines showed no such increase (E). For Q-PCR experiments, we used a pool of five fish per time-point; D2HG and L2HG measurements were averages of two replicates using 5 fish per replicate. Scale bar: 200 μm.

Expression Data

Gene:	EGFP
Fish:	zf3033Tg/zf3033Tg (TL) zf3035Tg/zf3035Tg (TL)
Anatomical Terms:	brain spinal cord
Stage Range:	Prim-5 to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf3035Tg/zf3035Tg (TL)
Observed In:	whole organism (R)-2-hydroxyglutarate(2-)
Stage Range:	Prim-5 to Day 6

Phenotype Detail

Acknowledgments

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