FIGURE

Fig. 7

ID

ZDB-FIG-180823-18

Publication

Mendelson et al., 2017 - The ceramide synthase 2b gene mediates genomic sensing and regulation of sphingosine Levels during zebrafish embryogenesis

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Fig. 7

Transcriptional changes during oogenesis underlie sphk2^MZ resistance to sphingosine buildup.

Oocytes isolated from wildtype and sphk2^-/- female fish show (A) increased sphingosine (Sph) levels in mutant oocytes associated with loss of S1P (Sph-1P) with (B) no difference in total ceramide levels. (C) Oocytes derived from sphk2^-/- oocytes showed significant upregulation of cers2b transcripts, as imaged following in situ hybridization. Note that signal in the wildtype oocytes was equivalent to background staining using a sense-strand control probe. (D) Correspondingly, embryos derived from sphk2^-/- females (right) showed relative tolerance to sphingosine accumulation when compared to those from wildtype females (left) when cultured in medium with 5 uM sphingosine. For (A, B) . results are averaged from three independent experiments using at least 50 embryos per measurement. For (C,D), representative embryos are shown, n > 25, from at least three independent experiments.

Expression Data

Gene:	cers2b
Fish:	WT sphk2^wcm2/wcm2
Anatomical Term:	oocyte
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT sphk2^wcm2/wcm2 sphk2^wcm2/+
Condition:	chemical treatment by environment: sphingosine
Observed In:	epiboly oocyte oocyte ceramide oocyte sphingoid 1-phosphate oocyte sphingosine yolk
Stage Range:	50%-epiboly to Adult

Phenotype Detail

Acknowledgments

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