Fig. 3

NF-kB-dependent inflammation is present, but p53-dependent apoptosis is not required for EtOH-induced fiber detachments. (A1–B6) Anterior left, dorsal top, side-mounted 72 hpf Tg(NF-kB:EGFP) embryos stained with phalloidin (pseudo-colored fuchsia) to visualize actin. (A1–6) 72 hpf Tg(NF-kB:EGFP) control embryo. (A1–2) Phalloidin channel only. (A2) Zoomed-in view corresponds to white box in A5. (A3–4) GFP channel only. (A4) Zoomed-in panel corresponds to white box in A5. Note low NF-kB activity in muscle fibers. (A5) Phalloidin and NF-kB-EGFP channels merged. (A6) Zoomed-in panel corresponds to white box in A5. (B1–6) 72 hpf Tg(NF-kB:EGFP) embryo treated continuously with 2% EtOH from 30 to 72 hpf. (B1–2) Phalloidin channel only. (B2) Zoomed-in view corresponds to white box in B5. (B3–4) GFP channel only. (B4) Zoomed-in view corresponds to white box in B5. Note increased NF-kB activity in muscle fibers due to EtOH exposure. White arrows points to muscle fibers expressing GFP. (B5–6) Phalloidin and NF-kB-EGFP channels merged. (B6) Zoomed-in view corresponds to white box in B5. (C–D) Quantification of fiber detachment frequency within and between AB or tp53 mutant embryos treated with EtOH from 30 to 72 hpf. (C) Percent of EtOH-treated embryos displaying one or more fiber detachments. There was no significant difference in fiber detachment frequency between embryos depending on AB (gray bars) vs. tp53 mutant (white bars) genetic background at 1% EtOH. (D) Percent of imaged segments with fiber detachments per embryo. There was no significant difference in fiber detachment frequency within embryos depending on AB (gray bars) vs. tp53 mutant (white bars) genetic background at 1% EtOH. Scale bars are 50 micrometers. “e” = embryo. Error bars are standard error of the mean.