FIGURE

Fig. 2

ID

ZDB-FIG-180703-47

Publication

Bambino et al., 2017 - Inorganic arsenic causes fatty liver and interacts with ethanol to cause alcoholic liver disease in zebrafish

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Fig. 2

Zebrafish are capable of arsenic metabolism and accumulate iAs in their tissues. (A) Expression of the zebrafish as3mt transcript is dynamic during zebrafish development, as determined by qRT-PCR. as3mt is maternally provided. Expression is enriched in the liver at 120 hpf. Error bars correspond to mean±s.d. L, liver; C, carcass. (B) Representative images of LA-ICP-MS analysis of 10-μm sections of control and iAs-exposed larvae. Following exposure from 4 to 120 hpf, iAs accumulated in the eye (white arrows, white circle in enlarged image), liver (yellow arrows, yellow circle in enlarged image) and in the gut (green arrows, green circle in the enlarged image). Refer to Table S2 for operating parameters. (C) Quantification of total arsenic content in the livers of 5-dpf larvae by ICP-MS. Livers dissected from larvae exposed to 0, 0.1, 0.5 and 1.0 mM iAs from 4 to 120 hpf showed a dose-dependent increase in the total arsenic content per liver (n=3 clutches). Error bars correspond to mean±s.d. hpf, hours post-fertilization; LA-ICP-MS, laser-ablation–inductively-coupled-plasma–mass-spectroscopy; iAs, inorganic arsenic; ICP-MS, inductively-coupled-plasma–mass-spectroscopy.

Expression Data

Gene:	as3mt
Fish:	WT
Anatomical Terms:	liver whole organism
Stage Range:	1-cell to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	chemical treatment by environment: sodium arsenite
Observed In:	eye sodium arsenite gut sodium arsenite liver sodium arsenite
Stage:	Day 5

Phenotype Detail

Acknowledgments

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