Fig. 1

Exposure to iAs is toxic to zebrafish embryos. (A) Survival analysis of zebrafish treated with iAs from 4 hpf through 6 dpf. Fish were scored daily for mortality (n≥2 clutches, >80 embryos per condition, Table S1). Red arrow indicates addition of iAs. Concentration of iAs used throughout the manuscript is shown in red and indicated by an asterisk. (B) Bright-field images of representative wild-type control (top) and arsenic-exposed (bottom) 5 dpf zebrafish larvae. Arrows indicate arsenic-induced phenotypes, including shortened body length, edema, clustering of pigment cells, under-consumption of yolk, and a small head. (C) Exposure to increasing doses of iAs from 4 hpf to 5 dpf led to an accumulation of phenotypes. The proportion of surviving embryos at 5 dpf that were affected increased with increasing concentrations of iAs (n=2 clutches exposed to 0.1 mM or 0.3 mM, n=3 clutches exposed to 0.6 mM, 1.0 mM or 1.4 mM, >30 fish exposed per treatment condition, Table S1). hpf, hours post-fertilization; dpf, days post-fertilization; iAs, inorganic arsenic.