Fig. 3
Long-Term Extension of Myelin Sheaths Compensates for Body Growth (A) Photographs of zebrafish at 4 and at 28 days post-fertilization (dpf). Scale bar, 1 mm. (B) Quantification of the relative increase of sheath length from their respective initiation onward compared to the increase in somite length over the same time. Data are expressed as mean ± SD. (B′) Quantification shown in (B) corrected for increase in body growth. Data are expressed as median with interquartile range (Wilcoxon signed-rank test). (C) Correlation between measured sheath length and their predicted length using increase in somite size as a reference. (D–D′′′) Timeline of confocal images of an individual cntn1b:mCherry, cntn1b:NFacsa-EYFP co-expressing neuron in the zebrafish spinal cord between 5 and 14 dpf. Scale bar, 100 μm (D and D′). (D″) Quantification of sheath lengths along the axon shown in (D) between 5 and 14 dpf. (D′′′) Quantification shown in (D″) corrected for animal body growth. See also Figure S2 and Movie S2. |