Fig. 9

Mosaic misexpression ofsox2orsox3. (A-B′) Expression of pax8 at 11 hpf in a wild-type host embryo into which fluorescent dextran-labeled hs:sox2/+ transgenic cells were transplanted. The embryo was heat shocked at 9 hpf, 39 °C for 60 min. Bright-field (A, A′) and fluorescent (B, B′) images of the same specimen. An enlargement of the left otic placode (A′, B′) shows the positions of transgenic cells (arrows). (C, D) Bright-field (C) and fluorescent (D) images showing pax2a expression at 14 hpf in a wild-type host embryo with fluorescent dextran-labeled hs:sox2/hs:sox2 transgenic cells (indicated by arrows). The embryo was heat shocked at 12 hpf, 39 °C for 60 min. (E, F) Bright-field (E) and fluorescent (F) images showing pax8 expression in the otic placode at 11 hpf in a wild-type host embryo with fluorescent dextran-labeled hs:sox3/+ transgenic cells. The embryo was heat shocked at 9 hpf, 39 °C for 60 min, and the positions of transgenic cells are indicated (arrows). (G-H′) Bright-field (G, G′) and fluorescent (H, H′) images showing pax2a expression at 11 hpf in a wild-type host embryo with fluorescent dextran-labeled hs:sox3/+ transgenic cells. The embryo was heat shocked at 9 hpf, 39 °C for 60 min. An enlargement of the left otic placode (G′, H′) shows positions of transgenic cells (arrows). (I, J) Bright-field (I) and fluorescent (J) images showing pax2a expression at 14 hpf in a wild-type host embryo with fluorescent dextran-labeled hs:sox3/+ transgenic cells. The embryo was heat shocked at 12 hpf, 39 °C for 60 min. Positions of transgenic cells are indicated (arrows).