FIGURE

Fig. 2

ID

ZDB-FIG-180608-69

Publication

Eve et al., 2017 - Knockdown of Laminin gamma-3 (Lamc3) impairs motoneuron guidance in the zebrafish embryo

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Fig. 2

Lamc3 knockdown embryos do not develop the parachordal chain (PAC).

(A–B) Lateral view of the zebrafish trunk stained using anti-LAMC3 antibody shows reduction in Lamc3 protein at 50 hpf in knockdown embryos (E, n=10) compared to standard controls (D, n=11). (C–I) Vasculature of 48 hpf Tg(fli1a:egfp) embryos. (C) Embryos injected with standard control MO develop the PAC. (D) Lamc3 knockdown embryos do not develop the PAC. (E) An enlarged image of the trunk vasculature in control embryos the PAC is indicated by a white arrowhead. (F) An enlarged image of the trunk vasculature in Lamc3 SBMO injected embryos. (G) The parachordal chain in control embryos at 72 hpf (white arrowhead). (H) By 72 hpf Lamc3 embryos have still not developed a parachordal chain. (I) Quantification of all ISVs with a PAC in Tg(fli1a:egfp) uninjected (white, n=545), control (green, n=462), Lamc3 SBMO knockdown (magenta, n=1133) and Lamc3 TBMO knockdown embryos (blue, n=880) at 48 hpf and 72 hpf, represent

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hu4624Tg; s896Tg + MO1-lamc3 + MO4-tp53 nim5Tg; y1Tg + MO1-lamc3 + MO4-tp53 sd2Tg; y1Tg + MO1-lamc3 + MO4-tp53 sd2Tg; y1Tg + MO2-lamc3 + MO4-tp53
Knockdown Reagents:	MO1-lamc3 MO2-lamc3 MO4-tp53
Observed In:	intersegmental artery intersegmental vein intersegmental vessel parachordal vessel parachordal vessel posterior cardinal vein sprouting angiogenesis thoracic duct
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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