FIGURE

Fig. 7

ID

ZDB-FIG-180417-34

Publication

Rogers et al., 2017 - Nodal patterning without Lefty inhibitory feedback is functional but fragile

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Fig. 7

Expression of lefty2 responds sensitively to Nodal signaling perturbations.

Wild type embryos at the one-cell stage were injected with 0.5 pg gfp mRNA (first row, (A–B’)), 0.5 pg lft1 mRNA (second row, (C–D’)), 7.5 pg gfp mRNA (third row, (E–F’)), or 7.5 pg CA-smad2 mRNA (fourth row, (G–H’)). Embryos were fixed at 50% epiboly and shield stages and expression of endogenous lft2 (A,B,C,D,E,F,G,H) and the mesoderm marker noto (A’,B’C’,D’,E’,F’,G’H’) were assessed using in situ hybridization. Note the dramatic decrease and increase in lft2 expression after lft1 (A,B,C,D) and CA-smad2 injection (E,G,F,H), respectively. Expression of noto was relatively unchanged in all treatments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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