FIGURE

Fig. 6-S4

ID

ZDB-FIG-180417-33

Publication

Rogers et al., 2017 - Nodal patterning without Lefty inhibitory feedback is functional but fragile

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6-S4

Nodal activity gradients in under- and over-rescued lefty double mutants.

To examine the tolerance to variation in Nodal signaling gradients, lft1^-/-;lft2^-/- embryos were exposed to 1 µM (‘under-rescued’, (A–A’’’)), 2 µM (‘rescued’, (B–B’’’)), or 3 µM (‘over-rescued’, (C–C’’’)) of the Nodal inhibitor drug SB-505124 beginning at the 8 cell stage. Embryos were fixed at 50% epiboly and stained for pSmad2 as described in the Materials and methods section. The under-rescue treatment leads to marked expansion of the Nodal signaling gradient (A’, A’’) and phenotypes at 1 day post-fertilization (dpf) consistent with hyperactive Nodal signaling (A’’’). The over-rescue treatment leads to a Nodal gradient with reduced amplitude (C’, C’’) and phenotypes consistent with mild Nodal loss of function (e.g. cyclopia) (C’’’). Images in A’’, B’’, and C’’ are cropped as indicated from A’, B’, and C’, respectively. Note that rescuing concentrations of SB-505124 vary from batch to batch, but are internally consistent within each experiment.

Expression Data

Antibody:	Ab9-smad2
Fish:	lft2^a146/a146; lft1^a145/a145
Condition:	chemical treatment by environment: SB 505124
Anatomical Term:	whole organism
Stage:	50%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife