FIGURE

Fig. S3

ID

ZDB-FIG-180202-4

Publication

Joris et al., 2017 - Number of inadvertent RNA targets for morpholino knockdown in Danio rerio is largely underestimated: evidence from the study of Ser/Arg-rich splicing factors

Other Figures

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Fig. S3

srsf5a/Δ36 cannot replace the wildtype mRNA srsf5a production. A, Expression of the Flag-tagged proteins was determined by Western blot analysis using the anti-Flag antibodies. Proteins were extracted from 24hpf uninjected embryos (NI) or injected with 50 pg of GFP-Flag, 100 pg of srsf5a-Flag, 100/200 pg of srsf5a/Δ36-Flag RNAs. Srsf5a-Flag could be visualized from 100pg of injected RNAs while Srsf5a/Δ36-Flag could be visualized when 200 pg is injected. B, Images represent uninjected embryos or injected with 100 pg of srsf5a RNAs or 200 pg of srsf5a-Δ36 RNAs at 48hpf. srsf5a injected embryos present developmental defects.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.