FIGURE

Fig. 4

ID

ZDB-FIG-180202-12

Publication

McCammon et al., 2017 - The 16p11.2 homologs fam57ba and doc2a generate certain brain and body phenotypes

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Body size and lipid amounts are affected by doc2a and fam57ba. A representative experiment is shown from the 5 conducted. (A,B) 12 dpf larvae stained with Oil Red O (ORO) are shown for wild-type and doc2a^+/− fam57ba^+/−. (C) A significant increase in body length as measured from the tip of the nose to the end of the tail is found for doc2a^+/− fam57ba^+/− larvae (n = 19) compared to wild-type (n = 19). (D) ORO was extracted back out of the stained larvae and quantified, showing a significant increase in the concentration of lipids in the double heterozygotes (yellow bar) compared to wild-type (blue bar). (E) After accounting for length, there is no longer a significant increase in ORO concentration in doc2a^+/− fam57ba^+/− larvae. (F,G) Wild-type and fam57ba^−/− larvae stained with ORO. (H) A significant increase in body length was found comparing fam57ba^−/− larvae (n = 30) to wild-type (n = 30). (I) ORO quantification showed a significant increase in the concentration of lipids for fam57ba^−/− homozygotes (purple bar) compared to wild-type (blue bar). (J) When accounting for length, ORO concentration is still significantly elevated in fam57ba^−/− fish. (K–Y) No significant changes in size or ORO concentration were observed for fam57ba^+/− (n = 29), doc2a^+/− (n = 26), and doc2a^−/− (n = 36) larvae compared to their respective wild-type controls. **P < 0.01, ***P < 0.001, ****P < 0.0001. Scale bar = 500 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	doc2a^wi721/wi721 tlcd3ba^wi720/wi720 doc2a^wi721/+ tlcd3ba^wi720/+ doc2a^wi721/+; tlcd3ba^wi720/+
Observed In:	whole organism whole organism lipid
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Hum. Mol. Genet.