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Fig. 4
Body size and lipid amounts are affected by doc2a and fam57ba. A representative experiment is shown from the 5 conducted. (A,B) 12 dpf larvae stained with Oil Red O (ORO) are shown for wild-type and doc2a+/− fam57ba+/−. (C) A significant increase in body length as measured from the tip of the nose to the end of the tail is found for doc2a+/− fam57ba+/− larvae (n = 19) compared to wild-type (n = 19). (D) ORO was extracted back out of the stained larvae and quantified, showing a significant increase in the concentration of lipids in the double heterozygotes (yellow bar) compared to wild-type (blue bar). (E) After accounting for length, there is no longer a significant increase in ORO concentration in doc2a+/− fam57ba+/− larvae. (F,G) Wild-type and fam57ba−/− larvae stained with ORO. (H) A significant increase in body length was found comparing fam57ba−/− larvae (n = 30) to wild-type (n = 30). (I) ORO quantification showed a significant increase in the concentration of lipids for fam57ba−/− homozygotes (purple bar) compared to wild-type (blue bar). (J) When accounting for length, ORO concentration is still significantly elevated in fam57ba−/− fish. (K–Y) No significant changes in size or ORO concentration were observed for fam57ba+/− (n = 29), doc2a+/− (n = 26), and doc2a−/− (n = 36) larvae compared to their respective wild-type controls. **P < 0.01, ***P < 0.001, ****P < 0.0001. Scale bar = 500 µm.