FIGURE

Fig. 4

ID

ZDB-FIG-180201-14

Publication

Chen et al., 2017 - Improving the Delivery of SOD1 Antisense Oligonucleotides to Motor Neurons Using Calcium Phosphate-Lipid Nanoparticles

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Fig. 4

Visualization of CaP-lipid NPs and neurons in the spinal cord and brain of a 6-dpf zebrafish. Sterile filtered non ASO-loaded CaP-lipid NPs containing LissRdB-DSPE (red) were micro injected into the zebrafish spinal cord (A) and brain (B). (A) Visualization of CaP- lipid NPs containing LissRdB-DSPE (red; injections) within the spinal cord neurons (green; transgenic expression) of a 6-day-old transgenic zebrafish. Zoomed image of the treated zebrafish from area indicated by white box, showing distribution of the CaP-lipid NPs within GFP-expressing spinal cord neurons. (B) Expression of brain-injected CaP-lipid NPs (red) in a transgenic zebrafish expressing astrocyte-specific GFP [green; Tg(GFAP:GFP)] to highlight the brain-specific delivery of these particles. The bottom image overlays the bright-field channel for better visualization of the CNS. The schematic inserts in panels depict the orientation of the fish and outline the presented area.

Expression Data

Gene:	GFP
Fish:	mi2001Tg
Anatomical Term:	astrocyte
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Neurosci.