FIGURE

Fig. 6

ID

ZDB-FIG-180123-5

Publication

Chang et al., 2017 - Metformin Inhibits Cyst Formation in a Zebrafish Model of Polycystin-2 Deficiency

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Fig. 6

Metformin cannot rescue the cystic phenotype in tsc1a morphants. Tg(wt1b:GFP) tsc1a morphants were immersed in embryo media (E3) containing 2.5 to 20 mM metformin until 48 hpf. (A) Representative images indicating prominent cystic pronephros (arrows) in Tg(wt1b:GFP) tsc1a morphants. Metformin does not affect the formation and severity of pronephric cysts. The upper panels show fluorescence images (dorsal view, anterior to the top). The lower panels show overlays of fluorescence (green) and transmission (grey) images of the upper panels. Scale bar, 100 µm. (B) Comparison of the cystic phenotype in different treatment groups. Data represent two independent experiments (n = 28–46 per group). ***P < 0.001, n.s. = not significant.

Expression Data

Gene:	EGFP
Fish:	li1Tg li1Tg + MO1-tsc1a
Condition:	chemical treatment by environment: metformin
Knockdown Reagent:	MO1-tsc1a
Anatomical Term:	pronephros
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	li1Tg + MO1-tsc1a
Condition:	chemical treatment by environment: metformin
Knockdown Reagent:	MO1-tsc1a
Observed In:	pronephros pronephros morphogenesis
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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