Fig. 7
- ID
- ZDB-FIG-180119-32
- Publication
- Eneman et al., 2017 - Pituitary adenylate cyclase-activating polypeptide (PACAP) in zebrafish models of nephrotic syndrome
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Quantification of thrombocytes in adriamycin treated zebrafish. (A) LEFT: GFP-labeled thrombocytes and thrombocyte precursors are formed in the zebrafish caudal hematopoietic tissue (CHT). Representative pictures of the CHT region at 4 dpf of embryos exposed to adriamycin 0, 10 and 30 μM are shown. No obvious difference in thrombocytes was observed between the controls and the adriamycin exposed fish. RIGHT: Pixel intensity was measured using ImageJ software. The graph represents means ± SD from measurements in three embryos per condition. No significant difference was observed between adriamycin exposed and control embryos. (B) LEFT: Western blot for GFP and β-actin (loading control) was performed using total zebrafish lysates at 4 dpf. A representative blot is shown. RIGHT: Signal intensity was measured using ImageJ software. The graph represents means ± SD from measurements in two repeated experiments. No significant difference was observed between adriamycin exposed and control embryos. |
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| Stage: | Day 4 |
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| Stage: | Day 4 |