Tgfb1 promotes serotonin synthesis and krasV12-induced carcinogenesis. Three-month-old adult zebrafish were treated for 7 days with 30 μg/mL dox with or without 2 μmol/L serotonin, BW23C86, PCPA, and SB204741. (A) IF staining of serotonin in liver section of these zebrafish. These slides also were co-stained for hepatocytes (HNF4a) and nuclei (4′,6-diamidino-2-phenylindole [DAPI]). Right: Quantification of serotonin-positive liver cells as percentages of hepatocytes (n = 10). Three-month-old Male krasV12-expressing zebrafish were treated with dox with or without SB204741 and SB431542, respectively. (B) Gross morphology and H&E staining of liver sections of these zebrafish. Quantification of tumor histology observed in the H&E-stained liver sections of these zebrafish (n > 8 in each group). (C–H) IF staining and quantification of (C) PCNA+, (D) caspase 3+, (E) P-Erk+, (F) P-Smad2, (G) P-Tph+, and (H) serotonin+ cells in liver sections (n > 8 in each group). *P < .05. Scale bars: (B) 3 mm (toprow) and 50 μm (bottom row), and (A, C–H) 20 μm.
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