FIGURE

Fig. 2

ID

ZDB-FIG-171229-2

Publication

Shainer et al., 2017 - Novel hypophysiotropic AgRP2 neurons and pineal cells revealed by BAC transgenesis in zebrafish

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Fig. 2

AgRP2 is expressed in uncharacterized pineal cells.

(a) Pineal gland of a 5-dpf agrp2:mCherry, Tg(aanat2:EGFP)^y9 larva. AgRP2 cells (magenta) do not co-localize with pineal photoreceptor cells (green). (b) Pineal gland of a 5-dpf agrp2:mCherry, Tg(foxd3:EGFP)^zf104 larva. AgRP2 cells (magenta) do not co-localize with FoxD3 pineal neurons (green). (c) Double immunostaining of a 5-dpf agrp2:mCherry larva with an antibody against HuC (a neuronal marker) and with anti-RFP. AgRP2 cells (magenta) do not co-localize with HuC-positive cells (green). (d) Immunostaining of a 5-dpf agrp2:EGFP larva with an antibody against GFAP (a marker for pineal interstitial cells) and with anti-EGFP. AgRP2 cells (magenta) do not co-localize with pineal interstitial cells (green). Scale bar, 10 μm.

Expression Data

Genes:	EGFP mCherry
Antibody:	Ab11-gfap
Fish:	tlv06Tg c264Tg; tlv05Tg; y9Tg c264Tg; tlv05Tg; zf104Tg
Anatomical Terms:	epiphysis epiphysis neuron epiphysis photoreceptor cell
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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