Fig. S6

Morpholino (MO) knockdown of two pycr genes in zebrafish caused severe defects during embryonic development. (A) Two pycr MOs (5 or 7.5 ng of each MO) were co-injected into wild-type zebrafish embryos at the 1- to 2-cell stage. Lateral views of representative wild-type control (Ctrl) or embryos coinjected with pycr1 and pycr2 MOs, pycr1 and pycr3 MOs or pycr2 and pycr3 MOs were photographed at 24 hours post fertilization (hpf), 24 hpf and 48 hpf respectively. (left panel: low magnification; right panel: high magnification) Co-injection with two pycr MOs resulted in reduced body length, a curly tail or abnormal somite phenotypes at 48 hpf. The control embryo exhibited chevron-shaped somites, but flatter morphology and shorter somites were observed in pycr MOs-injected embryos. The dash-line depicts the shape of somites. Scale bars: 100 µm. (B) Phenotypic distributions for embryos coinjected with two pycr MOs. For all co-injections, we observed a marked increase in the percentage of affected embryos with severe phenotypes.