FIGURE

Fig. S5

ID

ZDB-FIG-171228-2

Publication

Kuo et al., 2016 - PYCR1 and PYCR2 Interact and Collaborate with RRM2B to Protect Cells from Overt Oxidative Stress

Other Figures

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Fig. S5

Morpholino (MO) knockdown of pycr in zebrafish affects somatogenesis and cerebral hemorrhage during embryonic stages. (A) Each pycr MO (7.5 ng) was injected into wild-type zebrafish embryos at the 1- to 2-cell stage and evaluated at 48 hours post fertilization (hpf). The major defects of pycr1-morphants (left panel) or pycr3-morphants (right panel) were reduced body length and a curly tail. The pycr2-MO injected embryos exhibited no obvious abnormality, but the morphants shows cerebral hemorrhage (middle panel). Arrows indicate area of hemorrhage. Lateral views of representative wild-type control (Ctrl) or MO-injected fish were photographed at 48 hpf. Bar: 100 µm. (B) Phenotypic distributions of pycr1, pycr2 and pycr3 morphants.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-pycr1a WT + MO1-pycr1b WT + MO1-pycr3
Knockdown Reagents:	MO1-pycr1a MO1-pycr1b MO1-pycr3
Observed In:	cerebellum post-vent region whole organism
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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