FIGURE

Fig. 7 S2

ID

ZDB-FIG-171205-40

Publication

Poureetezadi et al., 2016 - Prostaglandin signaling regulates nephron segment patterning of renal progenitors during zebrafish kidney development

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Fig. 7 S2

Prostaglandin treatment shifts the distal early and restricts the distal late in sim1a morphants.

(A) Embryos were microinjected with the sim1a MO and treated with 100 µM dmPGE₂ or 1% DMSO from 4 hpf to 24 hpf. WISH was then used to label for the PCT (slc20a1a), DE (slc12a1), and DL (slc12a3) (purple) and the somites (smyhc1) (red) at the 24 hpf stage. Black bars indicate segment gene expression domain. Red scale bar, 70 µm. (B) Segments were quantified in triplicate with at least 20 embryos per control and experimental group. Data are represented as ± SD, significant by t test comparing each drug treatment to the corresponding DMSO control group, *p<0.05, **p<0.005, ***p<0.0005, where n.s. indicates not significant.

Expression Data

Genes:	slc12a1 slc12a3 slc20a1a
Fish:	TU + MO1-sim1a
Condition:	chemical treatment by environment: 16,16-dimethylprostaglandin E2
Knockdown Reagent:	MO1-sim1a
Anatomical Terms:	pronephric distal early tubule pronephric distal late tubule pronephric proximal convoluted tubule
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU + MO1-sim1a
Condition:	chemical treatment by environment: 16,16-dimethylprostaglandin E2
Knockdown Reagent:	MO1-sim1a
Observed In:	pronephric distal early tubule pronephric distal late tubule pronephric proximal convoluted tubule
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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