FIGURE

Fig. 3

ID

ZDB-FIG-171016-32

Publication

Vettori et al., 2017 - Glucocorticoids promote Von Hippel Lindau degradation and Hif-1α stabilization

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Fig. 3

Cross-talk between the HIF-1 and glucocorticoid-signaling pathways is mediated by the glucocorticoid receptor and HREs. (A) The 72-hpf Tg(4xhre-tata:eGFP)^ia21 embryos were incubated with different concentrations of DEX. DEX activates the 4xhre-tata transgene. (Magnification: 8×.) (B) Images of 72-hpf Tg(4xhre-tata:eGFP)^ia21 larvae injected with a gr-MO and a CTRL-MO alone or combined with DEX (Bottom). DEX activation of 4xhre-tata transgene expression is maintained in CTRL-MO–injected larvae and ablated in GR morphants. (Magnification: 8×.) (C) Histograms showing the average values (±SEM) of the fluorescence-integrated density in 72-hpf Tg(4xhre-tata:eGFP)^ia21 embryos injected with gr-MO and CTRL-MO with or without DEX. A.U., Arbitrary units. (D) Fold changes (±SEM) in phd3 gene expression in 72-hpf embryos injected with gr-MO and CTRL-MO with or without DEX (24 h) compared with nontreated/noninjected controls (set at 1). Target gene mRNA levels were normalized to β-actin. *P < 0.05; ***P < 0.001.

Expression Data

Genes:	EGFP egln3
Fish:	WT WT + MO4-nr3c1 ia21Tg ia21Tg + MO4-nr3c1
Condition:	chemical treatment: dexamethasone
Knockdown Reagent:	MO4-nr3c1
Anatomical Term:	whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO4-nr3c1 ia21Tg ia21Tg + MO4-nr3c1
Condition:	chemical treatment: dexamethasone
Knockdown Reagent:	MO4-nr3c1
Observed In:	whole organism
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA