ZFIN Image: Vettori et al., 2017, Fig. 3

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Figure Caption

Fig. 3

Cross-talk between the HIF-1 and glucocorticoid-signaling pathways is mediated by the glucocorticoid receptor and HREs. (A) The 72-hpf Tg(4xhre-tata:eGFP)^ia21 embryos were incubated with different concentrations of DEX. DEX activates the 4xhre-tata transgene. (Magnification: 8×.) (B) Images of 72-hpf Tg(4xhre-tata:eGFP)^ia21 larvae injected with a gr-MO and a CTRL-MO alone or combined with DEX (Bottom). DEX activation of 4xhre-tata transgene expression is maintained in CTRL-MO–injected larvae and ablated in GR morphants. (Magnification: 8×.) (C) Histograms showing the average values (±SEM) of the fluorescence-integrated density in 72-hpf Tg(4xhre-tata:eGFP)^ia21 embryos injected with gr-MO and CTRL-MO with or without DEX. A.U., Arbitrary units. (D) Fold changes (±SEM) in phd3 gene expression in 72-hpf embryos injected with gr-MO and CTRL-MO with or without DEX (24 h) compared with nontreated/noninjected controls (set at 1). Target gene mRNA levels were normalized to β-actin. *P < 0.05; ***P < 0.001.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA