Fig. 4

NC-derived tissues form abnormally in fscn1a-morphant embryos.

(A) Lateral and ventral views of 5 dpf tp53^zdf1 embryos injected with coMO or fscn1aMO and stained with Alcian blue. Numbers correspond to pharyngeal arches. Asterisk denotes arches that are severely reduced in size or absent. (B) Lateral views of 3 dpf tp53^zdf1 embryos analyzed by whole-mount ISH for th. Arrows denote th-positive neurons of sympathetic ganglia. (C) Lateral views of section of the gut in 4 dpf Tg(phox2b:gfp) embryos injected with tp53MO or tp53MO plus fscn1aMO. Arrows denote phox2b-positive enteric neurons. (D) Lateral views of trunk in 3 dpf Tg(ngn1:gfp) embryos injected with tp53MO or tp53MO plus fscn1aMO. Arrows in top panel highlight ngn1-positive NC-derived dorsal root ganglia (drg) and central nervous system (CNS)-derived Rohon-Beard neurons (rb). In lower panel, arrowhead and asterisk indicate misplaced and absent dorsal root ganglia, respectively. drg; dorsal root ganglia, rb; Rohan-Beard neurons, ye; yolk extension. In all panels, anterior is to the left. All experiments in this figure were performed independently at least three times with similar results. All scale bars in this figure = 100 μm.