Fig. S5

Regeneration of a large gap stripe pattern caused by ErbB inhibition.

(A-B) An eleven segment-wide gap in the first light stripe is filled initially be lateral movement of iridophores. (C-D) The new melanophores appear but in numbers lower than in the control segments. On 28 dpf in (C), an ectopic iridophore cluster is seen in the middle of the gap in segments numbered 7-8. (D-F) The iridophores from this new cluster begin to spread thus filling the gap completely on the right side (segments numbered 9-11) where melanophores also catch up in terms of numbers. Stripe pattern is restored in this region. On the left side of the gap (segments numbered 3-5), melanophores fail to appear in numbers comparable to that in the control regions and iridophores of abnormal morphology and organization appear. This region develops wavy stripes of abnormal width. Scale bar in A: 100 μm. (G) Bright field image of the same fish at adult stage, the locally abnormal stripe organization can still be seen (Scale bar=1 cm). Genotype: Tg(sox10:mRFP). Green: xanthophore auto-fluorescence. SL: standard length of fish.