FIGURE

Fig. S7

ID

ZDB-FIG-170815-27

Publication

Lim et al., 2017 - Caveolae Protect Notochord Cells against Catastrophic Mechanical Failure during Development

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Fig. S7

Tricaine washout experiment and touch-evoked response assay for cavin1b-/- embryos, related to Figure 6 and 7:

(A-B) Tricaine washout experiments represented by charts indicating cavin1b-/- lesion number (A) and severity index score (B). Cavin1b-/- embryos were either unanesthetized (purple) or anesthetized at 48 hpf and washed into E3 media at 62 hpf (blue) or 72 hpf (orange). P values were calculated using a two-way ANOVA with a posthoc Tukey’s multiple comparison test. 3 clutches per group. Number of fish used for all data groups>59.

(C-D) Chart showing the distance travelled by 4 and 5 dpf WT and cavin1b-/- embryos upon touch stimuli in measures of body length. Different clutches are represented by different dot colours (black, brown and blue). 3 clutches per group, number of individuals=45. P values were obtained using a two-tailed, unpaired t-test.

For (A-D), * P≤ 0.05; ** P≤ 0.01; *** P≤ 0.001; **** P≤ 0.0001. Data are presented as mean±SD.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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