FIGURE

Fig. 3

ID

ZDB-FIG-170815-16

Publication

Lim et al., 2017 - Caveolae Protect Notochord Cells against Catastrophic Mechanical Failure during Development

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Fig. 3

Notochord Lesions Are Damaged and Membrane-Permeable Cells

(A) Evans Blue Dye (EBD) infiltration into membrane-compromised notochord cells in live 55-hpf embryos. Arrows mark the mild lesion with corresponding specific EBD uptake. Note no uptake in skeletal muscles. Four fish per group are shown. The scale bar represents 100 μm.

(B) Alexa 488 5-UTP infiltration into membrane-compromised notochord cells in live 4-dpf embryos. Colocalization of Alexa 488 5-UTP with BODIPY TR methyl ester (endomembranes) occurred in moderate (middle row) and severe lesions (bottom row). No uptake in skeletal muscles was observed. Arrowheads mark lesions. n = 5 (WT) and n = 10 (mutant). The scale bar represents 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cavin1b^uq7rp/uq7rp
Observed In:	notochord inner cell plasma membrane skeletal muscle cell plasma membrane
Stage Range:	Long-pec to Day 4

Phenotype Detail

Acknowledgments

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