FIGURE

Fig. 2

ID

ZDB-FIG-170620-21

Publication

Vieira et al., 2017 - Repression of phosphatidylinositol transfer protein α ameliorates the pathology of Duchenne muscular dystrophy

Other Figures

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Fig. 2

Morpholino-mediated pitpnaa knockdown in zebrafish elicits dose-dependent changes in morphology and signaling. (A) Phase contrast images of sapje larvae injected with pitpnaa MO or control MO at 1 and 4 dpf. Embryos injected with 3 ng of pitpnaa MO at the one-cell stage showed abnormal development and were not viable, whereas 1 ng of pitpnaa MO did not negatively impact development. (B) Western blot showing decreased Pitpna expression with increasing doses of pitpnaa MO injected into sapje embryos. Protein lysates were harvested from sapje larvae at 4 dpf. Experiment performed in triplicate, n = 300 per replicate.

Expression Data

Gene:	pitpnaa
Antibodies:	Ab1-pitpna Ab1-ptena Ab5-akt
Fish:	dmd^{ta222a/ta222a} dmd^{ta222a/ta222a} + MO1-pitpnaa
Knockdown Reagent:	MO1-pitpnaa
Anatomical Term:	whole organism
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dmd^{ta222a/ta222a} + MO1-pitpnaa
Knockdown Reagent:	MO1-pitpnaa
Observed In:	whole organism
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA