FIGURE

Fig. S2

ID

ZDB-FIG-170614-2

Publication

Peng et al., 2017 - Fatty Acid Oxidation in Zebrafish Adipose Tissue Is Promoted by 1α,25(OH)₂D₃.

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Fig. S2

Expression levels of cyp27a1, cyp27b1 and cyp24a1 in the livers, calcium and phosphorus levels in plasma, and bone and cartilage. Related to Figure 2.

(A) QRT-PCR assays show the increased cyp27a1 and cyp27b1 transcript levels and diminished cyp24a1 transcript levels in the livers of mutants and wild type siblings at 100 dpf. (B) Calcium and (C) phosphorus levels of plasma were measured in the mutants and wild type siblings at 100 dpf. (D, E) Alcian Blue for cartilage and (F) Calcein for bone staining of cyp2r1^-/- zebrafish and the wild type siblings at 14 dpf. (G) MicroCT scanning of the mutants and control siblings at 100 dpf. The images are the representative result. D, lateral views; E, ventral views. The bars indicate means ± SD. *P< 0.05, **P< 0.01 versus controls, N.S., no statistically significant difference.

Expression Data

Genes:	cyp24a1 cyp27a1.4 cyp27b1
Fish:	WT cyp2r1^{ihb153/ihb153}
Anatomical Term:	liver
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cyp2r1^{ihb153/ihb153}
Observed In:	blood plasma calcium(2+) blood plasma phosphorus(1+) bone element cartilage element liver
Stage Range:	Days 14-20 to Adult

Phenotype Detail

Acknowledgments

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