Fig. 2
- ID
- ZDB-FIG-170605-21
- Publication
- Dzieciolowska et al., 2017 - Augmented quantal release of acetylcholine at the vertebrate neuromuscular junction following tdp-43 depletion
- Other Figures
- All Figure Page
- Back to All Figure Page
tardbpY220X/Y220X zebrafish embryos injected with an ATG tardbpl morpholino (MO) displayed significant morphological defects and reduced survival. A, Representative images of 2 dpf larvae from the following treatment groups: WT, WT + MO, tardbpY220X/+, tardbpY220X/Y220X, tardbpY220X/Y220X + Control MO (CoMo), tardbpY220X/Y220X + MO. Scale bar represents 1 mm, arrow indicates cardiac defect in the tardbpY220X/Y220X + MO treated larva. B, Eye diameter and C, body length was determined for each treatment group. tardbpY220X/Y220X + MO 2dpf larvae displayed significantly reduced eye diameter compared to all other treatment groups (p < 0.01). WT + MO, tardbpY220X/Y220X and tardbpY220X/Y220X + CoMo larvae displayed significantly reduced body length compared to WT larvae (p < 0.05) and tardbpY220X/Y220X + MO displayed significantly reduced body length compared to all treatment groups (p < 0.01). D, Quantification of gross morphological defects observed in all treatment groups indicating a higher incidence of defects in the tardbpY220X/Y220X + MO condition. E, Percentage survival curves of all treatment groups tracked over 11 days. tardbpY220X/Y220X + MO-treated larvae displayed reduced survival, with all animals dying by 10 dpf (p < 0.0001). N = 2, n = 50, *p < 0.05; **p < 0.01. |
| Fish: | |
|---|---|
| Knockdown Reagent: | |
| Observed In: | |
| Stage Range: | Long-pec to Days 7-13 |