ZFIN Image: Dzieciolowska et al., 2017, Fig. 2

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Fig. 2

tardbp^Y220X/Y220X zebrafish embryos injected with an ATG tardbpl morpholino (MO) displayed significant morphological defects and reduced survival.

A, Representative images of 2 dpf larvae from the following treatment groups: WT, WT + MO, tardbp^Y220X/+, tardbp^Y220X/Y220X, tardbp^Y220X/Y220X + Control MO (CoMo), tardbp^Y220X/Y220X + MO. Scale bar represents 1 mm, arrow indicates cardiac defect in the tardbp^Y220X/Y220X + MO treated larva. B, Eye diameter and C, body length was determined for each treatment group. tardbp^Y220X/Y220X + MO 2dpf larvae displayed significantly reduced eye diameter compared to all other treatment groups (p < 0.01). WT + MO, tardbp^Y220X/Y220X and tardbp^Y220X/Y220X + CoMo larvae displayed significantly reduced body length compared to WT larvae (p < 0.05) and tardbp^Y220X/Y220X + MO displayed significantly reduced body length compared to all treatment groups (p < 0.01). D, Quantification of gross morphological defects observed in all treatment groups indicating a higher incidence of defects in the tardbp^Y220X/Y220X + MO condition. E, Percentage survival curves of all treatment groups tracked over 11 days. tardbp^Y220X/Y220X + MO-treated larvae displayed reduced survival, with all animals dying by 10 dpf (p < 0.0001). N = 2, n = 50, *p < 0.05; **p < 0.01.

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Acknowledgments

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