FIGURE

Fig. 4

ID

ZDB-FIG-170602-35

Publication

Drerup et al., 2017 - Regulation of mitochondria-dynactin interaction and mitochondrial retrograde transport in axons

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Fig. 4

Mitochondrial retrograde transport is specifically disrupted in actr10 mutants.

(A,B) Stills from Videos 1 and 2 of mitochondrial transport in single pLL axons of a wildtype (A) and actr10 mutant (B). Yellow and red arrowheads point to mitochondria moving in the anterograde and retrograde directions respectively. (C,D) Kymograph analyses of mitochondrial transport in wildtype (C) and actr10 mutants (D). (E,F) Distance and velocity of mitochondrial transport in actr10 mutants (Ant-anterograde; Ret-retrograde; ANOVA). (G) The number of mitochondria moving in the retrograde direction is significantly reduced in actr10 mutants (ANOVA; p<0001). (H) The proportion of mitochondria moving in the retrograde, but not anterograde, direction is reduced in actr10 mutants (ANOVA with post-hoc contrasts; p<0.01). Scale bars = 10 µm. Error bars represent mean ± SEM. Number of larvae assayed (biological replicates) is indicated on graphs.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	actr10^nl15/nl15
Observed In:	posterior lateral line nerve anterograde axonal transport of mitochondrion posterior lateral line nerve retrograde axonal transport of mitochondrion
Stage:	Day 4

Phenotype Detail

Acknowledgments

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