FIGURE

Fig. 2

ID

ZDB-FIG-170602-33

Publication

Drerup et al., 2017 - Regulation of mitochondria-dynactin interaction and mitochondrial retrograde transport in axons

Other Figures

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Fig. 2

Loss of Actr10 causes mitochondrial accumulation in swollen axon terminals.

(A,B) At 4 dpf, swollen axon terminals in actr10 mutants (B) have high levels of Cytochrome c immunolabeling (arrow; NM3 shown) relative to a wildtype sibling (A). (C,D) TagRFP-labeled mitochondria accumulate in actr10 mutant (arrow), but not wildtype, axon terminals at 4 dpf. (E) Mean fluorescence intensity (background subtracted) of Cytochrome C in axon terminals of A and B. (F,G) Mean fluorescence intensity (background subtracted) in axon terminals of H-K show comparable levels of Lamp1 and pJNK fluorescence intensity between wildtype and actr10 mutant terminals (ANOVA; mean ± SEM shown). (H–K) Lamp1 and pJNK immunolabeling in NM3 axon terminals (arrows). Lamp1 and pJNK are in red in (H–K) and white in H'-K'. (L–O) Mitochondrial accumulation in mutant axon terminals, assayed using Cytochrome c immunolabeling (white), can be suppressed by mRNA-mediated expression of mRFP-Actr10. Arrows point to axon terminal regions for comparison. Scale bars = 10 µm.

Expression Data

Antibodies:	Ab1-cyc Ab1-lamp1 Ab15-mapk
Fish:	nl1Tg actr10^nl15/nl15; nl1Tg
Anatomical Terms:	posterior lateral line nerve axon terminus posterior lateral line neuromast axon terminus
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	actr10^nl15/nl15; nl1Tg
Observed In:	posterior lateral line nerve axon terminus posterior lateral line neuromast axon terminus
Stage:	Day 4

Phenotype Detail

Acknowledgments

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