Fig. S5

Dorsoventral patterning and Wnt activity in gpx4b mutants assessed by genotyping. (A) Representative expression of gsc and eve1 in progeny of allele1 homozygous female cross to heterozygous males at 6 hpf as revealed by WISH. Animal pole views with dorsal to the right. Arrows indicate the edges of the gsc and eve1 mRNA expression domains. (B) Genotyping results of individual embryos with indicated expression domain as shown in A. (C) Representative expression of cdx4 in progeny of allele1 homozygous female cross to heterozygous male at 80% epiboly as revealed by WISH. Asterisks indicate the edges of the cdx4 mRNA expression domains. (D) Genotyping results of individual embryos with indicated expression domain as shown in C. (E) Genotyping results of individual embryos with representative normal and dorsalized phenotypes (according to Figure 1D). (F) Quantification of genotyping results shown in E. (G) Genotyping results of individual embryos with representative normal and dorsalized phenotypes (according to Figure 1D). (H) Quantification of genotyping results shown in G. n = 5 females for allele1, n = 3 females for allele2. Scale bar = 200 μm. M, marker; Ht, heterozygous mutant; Mt, homozygous mutant.