PUBLICATION
Glutathione peroxidase 4 inhibits Wnt/β-catenin signaling and regulates dorsal organizer formation in zebrafish embryos
- Authors
- Rong, X., Zhou, Y., Liu, Y., Zhao, B., Wang, B., Wang, C., Gong, X., Tang, P., Lu, L., Li, Y., Zhao, C., Zhou, J.
- ID
- ZDB-PUB-170318-5
- Date
- 2017
- Source
- Development (Cambridge, England) 144(9): 1687-1697 (Journal)
- Registered Authors
- Liu, Yunzhang, Rong, Xiaozhi, Wang, Bo, Wang, Caixia, Zhao, Chengtian, Zhou, Jianfeng
- Keywords
- Glutathione peroxidase 4, Tcf/Lef, Wnt/β-catenin signaling, dorsal organizer, zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- CRISPR-Cas Systems/genetics
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/enzymology*
- Evolution, Molecular
- Gene Expression Regulation, Developmental
- Glutathione Peroxidase/chemistry
- Glutathione Peroxidase/deficiency
- Glutathione Peroxidase/metabolism*
- HEK293 Cells
- Humans
- Organizers, Embryonic/enzymology*
- Phenotype
- Promoter Regions, Genetic/genetics
- Protein Binding/genetics
- Selenium/metabolism
- Signal Transduction/genetics
- Transcription, Genetic
- Wnt Signaling Pathway*
- Zebrafish/embryology*
- Zebrafish/metabolism*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Zygote/metabolism
- PubMed
- 28302747 Full text @ Development
Citation
Rong, X., Zhou, Y., Liu, Y., Zhao, B., Wang, B., Wang, C., Gong, X., Tang, P., Lu, L., Li, Y., Zhao, C., Zhou, J. (2017) Glutathione peroxidase 4 inhibits Wnt/β-catenin signaling and regulates dorsal organizer formation in zebrafish embryos. Development (Cambridge, England). 144(9):1687-1697.
Abstract
The Wnt/β-catenin signaling pathway plays pivotal roles in axis formation during embryogenesis and in adult tissue homeostasis. Glutathione peroxidase 4 (GPX4) is a selenoenzyme and participates in the reduction of peroxides. Its synthesis depends on the availability of the element selenium. However, the roles of GPX4 in vertebrate embryonic development and underlying mechanisms are largely unknown. Here, we show that maternal loss of zebrafish gpx4b promotes embryonic dorsal organizer formation, whereas overexpression of gpx4b inhibits the development of the dorsal organizer. Depletion of human GPX4 and zebrafish gpx4b (GPX4/gpx4b) increases, while GPX4/gpx4b overexpression decreases, Wnt/β-catenin signaling in vivo and in vitro Functional and epistatic studies showed that GPX4 functions at the Tcf/Lef level, independently of selenocysteine activation. Mechanistically, GPX4 interacts with Tcf/Lefs and inhibits Wnt activity by preventing the binding of Tcf/Lefs to the promoters of Wnt target genes, resulting in inhibitory action in the presence of Wnt/β-catenin signaling. Our findings unravel GPX4 as a suppressor of Wnt/β-catenin signals, suggesting a possible relationship between the Wnt/β-catenin pathway and selenium via the association of Tcf/Lef family proteins with GPX4.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping