FIGURE

Fig. 3 S1

ID
ZDB-FIG-170517-36
Publication
Anelli et al., 2017 - Oncogenic BRAF disrupts thyroid morphogenesis and function via Twist expression
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Fig. 3 S1

Gene expression changes induced by BRAFV600E in larval thyrocytes.

(A) qPCR analysis of select up-regulated genes; data are represented as fold change of tg-BRAFV600E-TOM compared to tg-TOM and are normalized using gapdh as a control. (B) GSEA hallmark gene sets significantly enriched in tg-BRAFV600E-TOM (top 10 signatures, FDR < 25%, nominal p-value<5%). (C) GSEA enrichment plot of ERK signature using RNAseq data generated from sorted zebrafish thyrocytes as described. (D) Heat-map of ERK signature genes (n = 37) differentially expressed in tg-BRAFV600E-TOM sorted thyrocytes compared to tg-TOM. (E) Validation of differential expression of select ERK signature genes using qPCR, data are normalized using gapdh as a control. (F) E-cadherin immunostaining in tg-TOM and tg-BRAFV600E-TOM larvae at 5dpf.
Expression Data
Genes: (all 7)
Fish:
Anatomical Term:
Stage: Days 45-89

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Days 45-89

Phenotype Detail
Acknowledgments
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