FIGURE

Fig. 6

ID

ZDB-FIG-170516-52

Publication

Sauteur et al., 2017 - Distinct and redundant functions of Esam and VE-cadherin during vascular morphogenesis

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Fig. 6

Intrajunctional gaps are correlated with defects in apical polarization. (A-D) Deconvolved projections of SeAs of Tg(fli1a:EGFP)^y1 (blue) wild-type (A), esama^ubs19 (B), ve-cad^ubs8 (C) and esama^ubs19; ve-cad^ubs8 double mutant (dKO, D) embryos stained for Zo-1 (red) and Pdxl (green), around 32 hpf. SeAs are shown either in EGFP plus Zo-1 or in Pdxl plus Zo-1 channels, whereas insets and sections are merges of all three colors, except for (B″), where the blue channel was omitted. The panels on the right-hand side represent magnifications and cross-sections of the insets and dotted lines, respectively. Red arrowheads point to junctions; white arrowheads point to intrajunctional gaps or missing junctions between the white arrowheads in (D′); blue arrowheads point to basal localized Pdxl. L, lumen; asterisk, nucleus. Scale bars: 20 µm.

Expression Data

Genes:	EGFP podxl2
Antibodies:	Ab1-podxl2 Ab1-tjp1
Fish:	y1Tg cdh5^ubs8/ubs8; y1Tg esama^ubs19/ubs19; y1Tg cdh5^ubs8/ubs8; esama^ubs19/ubs19; y1Tg
Anatomical Terms:	angiogenic sprout apical plasma membrane angiogenic sprout tight junction intersegmental vessel intersegmental vessel basal plasma membrane intersegmental vessel tight junction
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cdh5^ubs8/ubs8; y1Tg cdh5^ubs8/ubs8; esama^ubs19/ubs19; y1Tg
Observed In:	cell-cell junction maintenance intersegmental vessel intersegmental vessel basal plasma membrane intersegmental vessel tight junction
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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