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Fig. 6
Intrajunctional gaps are correlated with defects in apical polarization. (A-D) Deconvolved projections of SeAs of Tg(fli1a:EGFP)y1 (blue) wild-type (A), esamaubs19 (B), ve-cadubs8 (C) and esamaubs19; ve-cadubs8 double mutant (dKO, D) embryos stained for Zo-1 (red) and Pdxl (green), around 32 hpf. SeAs are shown either in EGFP plus Zo-1 or in Pdxl plus Zo-1 channels, whereas insets and sections are merges of all three colors, except for (B″), where the blue channel was omitted. The panels on the right-hand side represent magnifications and cross-sections of the insets and dotted lines, respectively. Red arrowheads point to junctions; white arrowheads point to intrajunctional gaps or missing junctions between the white arrowheads in (D′); blue arrowheads point to basal localized Pdxl. L, lumen; asterisk, nucleus. Scale bars: 20 µm.