FIGURE

Fig. 5

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ZDB-FIG-170424-31

Publication

Zhang et al., 2017 - Myomaker is required for the fusion of fast-twitch myocytes in the zebrafish embryo

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Fig. 5

caRac is unable to induce robust ectopic fusion in myomaker^sq35 mutants. caRac can induce hyperfusion in non-mutant embryos (Top panel; arrow indicates a hyperfused caRac-expressing cell (labeled by anti-GFP staining)), but not in myomaker^sq35 mutants. Most caRac-expressing fast-twitch muscle cells in myomaker^sq35mutants are mononucleated (Bottom panel; arrow indicates an unfused cell), and a few cells are binucleated (Middle panel; triangles indicate the nuclei of a binucleated cell). All embryos are 2 d.p.f. and also stained with DAPI, anti-β-catenin and F310 antibodies to label nuclei, cell membranes and fast-twitch muscle cells, respectively. The bottom panel images are projections of 2 Z-sections. Scale bar=20 µm.

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Acknowledgments

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Reprinted from Developmental Biology, 423(1), Zhang, W., Roy, S., Myomaker is required for the fusion of fast-twitch myocytes in the zebrafish embryo, 24-33, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.