Fig. 4

Myomaker overexpression is sufficient to partially rescue myocyte fusion in myomaker^sq36 and jam2a^hu3319 mutants. A: Myomaker-expressing cells (as reported by anti-GFP staining) in myomaker^sq36mutant embryos can undergo fusion to form multinucleated fast-twitch muscle cells (Top panel; triangles indicate 3 nuclei within a Myomaker-expressing cell), or remain unfused (Bottom panel; arrow indicates a Myomaker-expressing mononucleated cell). Embryos are 36 h.p.f. and stained with DAPI, anti-β-catenin and F310 antibodies to label nuclei, cell membranes and fast-twitch muscle cells, respectively. Scale bar=20 µm. B: Myomaker overexpression can partially rescue myocyte fusion in jam2a^hu3319 mutants. Top panel depicts a binucleated Myomaker-expressing (reported by anti-GFP staining) fast-twitch muscle cell (nuclei indicated by triangles), and the bottom panel shows 3 mononucleated Myomaker-expressing fast-twitch muscle cells (indicated by arrows). All embryos are 36 h.p.f. jam2a^hu3319 mutants that have also been stained with DAPI, anti-β-catenin and F310 antibodies to label nuclei, cell membranes and fast-twitch muscle cells, respectively. Scale bar=20 µm.