ZFIN Figure: Carroll et al., 2014, Fig. 5

Fig. 5

Xenoestogens Partially Replicate the Effects of E2 on HSPC Formation

(A) Exposure to xenoestrogens ethinyl estradiol (EE), genistein (GEN), or Bisphenol A (BPA) decreased runx1/cmyb expression and could be partially blocked by cotreatment with FULV (n ≥ 25/treatment).

(B) Treatment with GEN reduced the expression of fli1/flk1 in the intersomitic vessels (n ≥ 25/treatment); an arrow points to ISVs.

(C) Treatment with GEN (15/25) and EE (12/23) decreased Notch:GFP expression.

(D) VEGFAa was noticeably decreased by EE (15/26); arrowheads indicate somite region.

(E) Summary of changes in the expression of markers of vascular identity ephrinB2, flt4, notch3, and deltaC following treatment with XEs (n ≥ 25).

(F) qPCR confirmed decreases in runx1, cmyb notch3, and scl by XE exposure (mean of triplicate experiments ± SEM; one-tailed t test ^∗p < 0.05; ^∗∗p < 0.01).

Expression Data

Genes:	EGFP kdrl myb notch3 runx1 tal1 vegfaa
Fish:	WT um14Tg
Conditions:	chemical treatment by environment: bisphenol A chemical treatment by environment: 17alpha-ethynylestradiol chemical treatment by environment: bisphenol A chemical treatment by environment: 17alpha-ethynylestradiol chemical treatment by environment: fulvestrant chemical treatment by environment: fulvestrant chemical treatment by environment: genistein
Anatomical Terms:	dorsal aorta intersegmental vessel somite ventral wall of dorsal aorta whole organism
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT um14Tg
Conditions:	chemical treatment by environment: bisphenol A chemical treatment by environment: 17alpha-ethynylestradiol chemical treatment by environment: bisphenol A chemical treatment by environment: 17alpha-ethynylestradiol chemical treatment by environment: fulvestrant chemical treatment by environment: fulvestrant chemical treatment by environment: genistein
Observed In:	dorsal aorta intersegmental vessel somite ventral wall of dorsal aorta whole organism
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 29, Carroll, K.J., Esain, V., Garnaas, M.K., Cortes, M., Dovey, M.C., Nissim, S., Frechette, G.M., Liu, S.Y., Kwan, W., Cutting, C.C., Harris, J.M., Gorelick, D.A., Halpern, M.E., Lawson, N.D., Goessling, W., North, T.E., Estrogen defines the dorsal-ventral limit of VEGF regulation to specify the location of the hemogenic endothelial niche, 437-53, Copyright (2014) with permission from Elsevier. Full text @ Dev. Cell