Fig. 5
Zebrafish functional screening of putative apoptotic regulators. A, Zebrafish embryos (flk1:EGFP-NLS embryos; green endothelial cell [EC] nuclei) were injected with morpholinos oligonucleotides (MOs) targeting candidate genes or a nontargeting control MO (indicated on the left of each row). EC apoptosis was studied in the presence (control MO) or in the absence (sih MO) of flow by whole-mount active caspase-3 staining (red). Apoptotic ECs (yellow) were monitored at 30 hours post fertilization (hpf). Lateral view, anterior to the left, dorsal up. Scale bar: 50 μm. B, The proportion of apoptotic ECs (number of apoptotic ECs divided by the total number of ECs) normalized to sih MO-injected embryos was calculated, and mean values are shown with SEM. n≥15 from 3 independent experiments. *P<0.05, **P<0.01, ***P<0.001 using 1-way ANOVA. |