IMAGE

Fig. 5

ID

ZDB-IMAGE-170222-12

Genes

EGFP

Antibodies

Ab4-casp3

Source

Figures for Serbanovic-Canic et al., 2017

Image

Figure Caption

Fig. 5

Zebrafish functional screening of putative apoptotic regulators. A, Zebrafish embryos (flk1:EGFP-NLS embryos; green endothelial cell [EC] nuclei) were injected with morpholinos oligonucleotides (MOs) targeting candidate genes or a nontargeting control MO (indicated on the left of each row). EC apoptosis was studied in the presence (control MO) or in the absence (sih MO) of flow by whole-mount active caspase-3 staining (red). Apoptotic ECs (yellow) were monitored at 30 hours post fertilization (hpf). Lateral view, anterior to the left, dorsal up. Scale bar: 50 μm. B, The proportion of apoptotic ECs (number of apoptotic ECs divided by the total number of ECs) normalized to sih MO-injected embryos was calculated, and mean values are shown with SEM. n≥15 from 3 independent experiments. *P<0.05, **P<0.01, ***P<0.001 using 1-way ANOVA.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Arterio., Thromb., and Vas. Bio.