Aldh1l1 and Ca2 abundance in WT and mutant fins. (A) Graphs of the relative abundance of aldh1l1 and ca2 transcripts in proximal compared with distal regions of uninjured fins determined by RT-qPCR. Data are normalized to the abundance of actb2 transcripts. Data are mean ± SEM of n = 6 experiments for aldh1l1 and n = 3 for ca2, with >8 fish per experiment. (B and C) Representative Western blots showing Aldh1l1 and Ca2 protein in the proximal (P), middle (M), and distal (D) regions of uninjured caudal fins (B) and uninjured pectoral and dorsal fins (C). Actb2 was used as a loading control. (D) Representative images of sof and lof uninjured caudal fins, with Western blots showing Aldh1l1 and Ca2 protein in the proximal (P), middle (M), and distal (D) regions of these mutant fins.
|