FIGURE

Fig. 3

ID

ZDB-FIG-170109-23

Publication

Takeuchi et al., 2015 - Type IV Collagen Controls the Axogenesis of Cerebellar Granule Cells by Regulating Basement Membrane Integrity in Zebrafish

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Fig. 3

The Col4a5 and Col4a6 complex plays a role in the axogenesis of GCs and RGCs.

Wild-type (A-E), col4a6^rk18/rk18 (F-J), and col4a5^s510/s510 (K-O) mutant 5-dpf larvae harboring the pou4f3:Gal4, UAS:GAP-GFP transgene, which labels the axons of retinal ganglion cells (RGCs) in a mosaic manner, were stained with anti-Vglut1 (magenta) and anti-GFP (green) antibodies. Dorsal projection views (A-C, F-H, K-M) of the midbrain/hindbrain (A, F, K), left half of the rostral hindbrain (B, G, L), and half of the tectum (C, H, M). Lateral view of the tectum (D, I, N). Both col4a6 and col4a5 mutant larvae showed abnormal branching of the GC bundles (indicated by arrows, G, L). In wild type animals, each RGC projected to a single layer in the tectum (D), whereas in the col4a6 and col4a5 mutants some RGC axons trespassed between tectal layers (marked by arrows in I and N). (E, J, O) Schematic drawing of the RGC axons (lateral view). SO, stratum opticus; SFGS, stratum fibrosum et griseum superficiale. The statistic analysis is shown in S2 Table. Scale bars: 100 μm in K (applied to A, F); 20 μm in L (applied to B, G); 20 μm in M (applied to C, H); 20 μm in N (applied to D, I).

Expression Data

Gene:	GFP
Antibody:	Ab1-slc17a7
Fish:	s318tTg col4a5^s510/s510; s318tTg col4a6^rk18/rk18; s318tTg
Anatomical Terms:	cerebellar granule cell axon cerebellar granule cell axon terminus retinal ganglion cell axon
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	col4a5^s510/s510; s318tTg col4a6^rk18/rk18; s318tTg
Observed In:	cerebellar granule cell axon extension involved in axon guidance retinal ganglion cell retinal ganglion cell axon guidance
Stage:	Day 5

Phenotype Detail

Acknowledgments

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