Fig. 1

There are three types of GCs in zebrafish cerebellum.

(A-D) Single-cell labeling was performed by crossing the GC-specific Gal4 line gSA2AzGFF152B and partially silenced UAS:Kaede reporter line (A, B, C), or by injecting UAS:Kaede reporter DNA in a Tol1 vector and Tol1 transposase RNA into the GC (in the LCa)-specific Gal4 line gSAIGFF23C (Fig 1D). Larvae at 5 dpf that expressed Kaede in one or a few GCs were selected, and Kaede was visualized by immunostaining with an anti-Kaede antibody. Dorsal projection views in the cerebellum. Co-staining with an antibody against Neurod, a GC marker, indicated that the Kaede⁺ cells in the cerebellum were GCs (A). (E) Schematic representation of the GC types. The GCs in the Va and CCe (rostromedial lobes) had a T-shaped axon, which formed parallel fibers with other GC axons, and targeted the dendrites of PCs (Ea). The GCs in the EG also had a T-shaped axon, which extended bilaterally and turned caudally to the dorsal hindbrain (Eb). The GCs in the LCa extended their axon only ipsilaterally, and it turned caudally. The GCs in the EG and LCa (caudolateral lobes) made synapses on the dendrites of PCs in the cerebellum and on those of crest cells (Cr) in the dorsal hindbrain (crista cerebellaris). Scale bars: 40 μm in A-D.