FIGURE

Fig. 3

ID

ZDB-FIG-161130-3

Publication

Grainger et al., 2016 - Wnt9a Is Required for the Aortic Amplification of Nascent Hematopoietic Stem Cells

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Fig. 3

Wnt9a Is Required for HSPC Formation

(A and B) WISH for wnt9a at 16.5 hpf, 19 hpf, and 24 hpf (A). Scale bars, 0.2 mm; representative images of n = 15. Imaged area is shown in (B).

(C and D) Representative images of kdrl:mCherry; cmyb:eGFP injected as listed (C). HSPCs per millimeter of aorta are quantified in (D). Scale bars, 30 μm.

(E) WISH for cmyb in 4 days post fertilization (dpf) fish treated with 0.1 ng or 1 ng of wnt9a MO. Scale bars, 0.2 mm.

(F) Wnt9a mutants were generated by injection of guide RNA targeting the last portion of the first exon.

(G and H) Expression of cmyb was examined by WISH in 36 hpf WT, wnt9a^+/Δ and wnt9a^Δ/Δ- zebrafish (G) and quantified in (H).

Scale bars, 100 μm. ^∗p < 0.05; ^∗∗∗p < 0.001; n.s., not significant. Error bars represent SD. See also Figure S3.

Expression Data

Genes:	EGFP mCherry myb wnt9a
Fish:	AB WT wnt9a^sd49/sd49 AB + MO2-wnt9a wnt9a^sd49/+ y171Tg; zf169Tg y171Tg; zf169Tg + MO1-wnt9a y171Tg; zf169Tg + MO1-wnt9b y171Tg; zf169Tg + MO2-wnt9a
Knockdown Reagents:	MO1-wnt9a MO1-wnt9b MO2-wnt9a
Anatomical Terms:	somite ventral wall of dorsal aorta hematopoietic stem cell
Stage Range:	14-19 somites to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	wnt9a^sd49/sd49 AB + MO2-wnt9a y171Tg; zf169Tg + MO1-wnt9a y171Tg; zf169Tg + MO1-wnt9b y171Tg; zf169Tg + MO2-wnt9a
Knockdown Reagents:	MO1-wnt9a MO1-wnt9b MO2-wnt9a
Observed In:	ventral wall of dorsal aorta hematopoietic stem cell
Stage Range:	Prim-25 to Day 4

Phenotype Detail

Acknowledgments

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