FIGURE

Fig. 2

ID

ZDB-FIG-160826-12

Publication

Ji et al., 2016 - Mutations in zebrafish pitx2 model congenital malformations in Axenfeld-Rieger syndrome but do not disrupt left-right placement of visceral organs

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Fig. 2

Functional analysis of mutant pitx2HD transcripts. (A) RNA in situ hybridizations revealed normal pitx2 mRNA expression in wild-type, pitx2HD^-/^- and pitx2c^-^/- embryos, including asymmetric pitx2c in lateral plate mesoderm (arrow) at the 18 somite stage (18 ss). L=left; R=right. (B) Microinjection of wild-type pitx2c mRNA into wild-type embryos resulted in severe developmental defects or lethality at 2 dpf, which was not frequently observed in controls injected with gfp mRNA. Most embryos injected with pitx2c mRNA containing the HD^sny15 mutation developed normally. n=number of embryos analyzed (pooled from three experiments).

Expression Data

Gene:	pitx2
Fish:	AB/TU pitx2^sny3/sny3 (AB/TU) pitx2^sny6/sny6 (AB/TU)
Anatomical Terms:	posterior lateral mesoderm left side prechordal plate
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 416(1), Ji, Y., Buel, S.M., Amack, J.D., Mutations in zebrafish pitx2 model congenital malformations in Axenfeld-Rieger syndrome but do not disrupt left-right placement of visceral organs, 69-81, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.