Fig. 2

Mutations in the Tbx4 NLS sequence cause the loss of pelvic fins in pelvic finless zebrafish. (A) Wild-type zebrafish (tbx4^+/+) pelvic fins are located on the posterior ventral flank of the fish, either side of the cloaca. (B) TALENs-induced mutated tbx4 causes the loss of pelvic fins in homozygous tbx4 mutation zebrafish (tbx4^gi1/gi1). (C) Heterozygous mutant tbx4 zebrafish (tbx4^gi1/+) display normal pelvic fin development. (D) Pelvic finless zebrafish (pfl^mq6/mq6) do not develop pelvic fins due to missense mutations in the Tbx4 NLS. (E) Pelvic finless; mutant tbx4 compound heterozygotes (tbx4^gi1/mq6) also do not develop pelvic fins. (F) Heterozygous wild-type; pelvic finless zebrafish (pfl^mq6/+) develop pelvic fins, confirming that the pelvic finless mutation is allelic to tbx4 and that the mutations in the NLS of Tbx4 (A78V; G79A) result in a loss of function of the tbx4 gene. White rectangle in A-F indicates zoomed in region shown in A′-F′. Scale bars: 1mm.